User Notes:

COLOR KEY:
- microtubules
- chromosomes/chromatin (DNA)
- callose

What's here

Each multi-color image is a composite of 2 or 3 black & white (b/w) images of the same specimen. The separate b/w images were captured through a microscope using a b/w CCD camera, then colorized in Photoshop and overlaid on one another.
    The reason the same specimen can produce different images is that it is "stained" with 1 or 2 or 3 different fluorescent dyes that can only be seen with specific combinations of illumination and viewing filters. Thus, the same specimen can be viewed different ways to reveal differently stained structures of the cells.
    Long-term goal is to describe what's shown. In the meantime, you can learn yourself from Dr NAD's Mini-Course (as well as Immunofluorescence Confocal Microscopy, Plant Microtubule Arrays).

Why click images

Click on clickable ones to see b/w originals. Need your browser's "back" button/command to return here because the b/w images are image files only, not html (no text, etc.).

Why see the b/w?

1)    Using monitor at 256 colors?
If so, these (jpeg) images aren't so pretty – probably grainy or fuzzy or speckled (called "dithered"). They can be better appreciated seeing the b/w.
2)    And using Netscape?
With any browser at 256 colors, these jpeg images can dither poorly. Even if these were gif images, which can be made not to dither, these would dither because they comprise so many color shades. Now, b/w images that are not on a page with other stuff ("in-line" images) sometimes don't dither so poorly.
    With Netscape, non-in-line gifs can show more without dithering, so the b/w to which these color images are linked will show you more detail. MicroSoft Internet Explorer (IE) users... sorry, Navigator is superior in this respect, unless your monitor is set at >256 colors, but the next item (3) is still true.
3)    To appreciate detail.
Finer topography details are discernible in the b/w images, and an appreciation for how the microscopy method is useful can be inferred.

How's it done?

Here are the steps in Adobe's Photoshop. And here are other web design tips from Dr NAD.


One more sample
©1996 Neil A Durso, III

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